albert-ying/autonomous-lab/bwa-mapping

Claude Code and agent skill by albert-ying/autonomous-lab

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Install

npx vskill@latest install albert-ying/autonomous-lab/bwa-mapping

SKILL.md

BWA Mapping Skill

When to Use

Use when mapping paired-end or single-end sequencing reads to a reference genome.

Standard Workflow

  1. Index reference genome: bwa index reference.fasta
  2. Map reads: bwa mem -t <threads> reference.fasta R1.fastq.gz R2.fastq.gz > aligned.sam
  3. Convert to sorted BAM: samtools sort -o aligned.sorted.bam aligned.sam
  4. Index BAM: samtools index aligned.sorted.bam

Key Decisions

  • Use BWA-MEM for reads >70bp (standard for Illumina HiSeq)
  • Add read group info with -R flag for downstream variant calling
  • Use appropriate thread count for available CPU cores

Security

  • Security scanPassed, 90 of 100Pattern checks on the skill's files.
  • Model reviewPassed

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