albert-ying/autonomous-lab/orthofinder

Claude Code and agent skill by albert-ying/autonomous-lab

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Install

npx vskill@latest install albert-ying/autonomous-lab/orthofinder

SKILL.md

OrthoFinder Skill

When to Use

Use OrthoFinder for identifying orthologous gene groups across multiple genomes, inferring gene trees, and identifying orthogroups (clusters of orthologous genes).

Standard Workflow

  1. Prepare protein FASTA files (one per genome) from gene predictions
  2. Run OrthoFinder: orthofinder -f <protein_fasta_dir>
  3. Parse results from Orthogroups/Orthogroups.tsv
  4. Filter orthogroups present in all species (core orthogroups)

Key Decisions

  • Use protein sequences (not nucleotide) for ortholog detection
  • Choose appropriate inflation parameter for MCL clustering
  • Filter single-copy orthologs for phylogenetic analysis

Security

  • Security scanPassed, 90 of 100Pattern checks on the skill's files.
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Source

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