r2ascension/skill_repo/bio-clip-seq-clip-motif-analysis

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Identify enriched sequence motifs at CLIP-seq binding sites for RBP binding specificity. Use when characterizing the sequence preferences of an RNA-binding protein.

Install

npx vskill@latest install r2ascension/skill_repo/bio-clip-seq-clip-motif-analysis

SKILL.md

The same SKILL.md was published earlier as freedomintelligence/openclaw-medical-skills/bio-clip-seq-clip-motif-analysis.

CLIP Motif Analysis

HOMER De Novo Motifs

bash
# Extract sequences from peaks
bedtools getfasta -fi genome.fa -bed peaks.bed -fo peaks.fa
# Find enriched motifs
findMotifs.pl peaks.fa fasta output_dir \
-len 6,7,8 \
-rna

MEME-ChIP

bash
meme-chip -oc output_dir \
-dna \
peaks.fa

Known Motif Enrichment

bash
# HOMER known motif scan
findMotifs.pl peaks.fa fasta output_dir \
-rna \
-known

Related Skills

  • clip-peak-calling - Get peaks
  • chip-seq/motif-analysis - General motif concepts

<!-- AUTHOR_SIGNATURE: 9a7f3c2e-MD-BABU-MIA-2026-MSSM-SECURE -->

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